Part:BBa_K2492003:Design

GEM-GECO, a calcium indicator. When bound with calcium, GEMgeco has blue shift of its emission.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1064
  Illegal SapI.rc site found at 970
  

Design Notes

We combine the channel with GEMgeco. The neuron is actived by lighting the channel, the concentration of calcium changes and cause the blue shift of GEMgeco. Thus, we can confirmed that the neuron is actived.

Source

Developed from green calcium indicator.